Raman Microspectroscopy In general terms, a Raman spectrometer is integrated with a Raman microscope. It produces a unique spectral fingerprint that identifies the chemical composition and molecular structure of materials. Timothy M. James, Simone Rupp, Helmut H. Telle. Energy 44, 31745–31756 (2019). New Raman spectroscopy applications are emerging in non-traditional fields because of advancements in easy-to-use commercial Raman spectroscopy instrumentation (1). Reinhall, and I.V. Advancements in the stability and portability of certain Raman systems demonstrate that this technology can be moved out of the traditional laboratory and installed at the point of need with little to no maintenance. For all compounds, FT‐Raman and two Vis‐Raman spectra are presented along with the detailed band assignment. Rasmussen, V. Maheshwari, J.C. Kramlich, P.G. If you are on a personal connection, like at home, you can run an anti-virus scan on your device to make sure it is not infected with malware. This article reviews two industrial applications that have successfully used Raman spectroscopy as a PAT tool: The first industrial application is the manufacture and sampling of highly sensitive and reactive trialkyl aluminum compounds. Raman Spectrometer Options for 532nm Laser excitation Request a Quote StellarNet offers a variety of high performance spectrometers configured for 532nm Raman spectroscopy applications that perform quick identification of a variety of liquid, solid, or powder samples. Given the ease of use, simplicity of data analysis, first principles compositional information, and versatility, Raman spectroscopy is an excellent tool for industrial process analysis and control. In some applications, the usage of two probes to monitor two production lines at the same time is possible with one system. The utility of Raman for in-line, real-time process monitoring and control is demonstrated for content uniformity. It is based on inline UV-Vis, NIR, and Raman spectroscopy. The BioPAT ® Spectro offers access to Raman spectroscopy analysis in high throughput process development, supporting faster Raman model building and accelerating scale-up into commercial manufacturing. The laboratory analysis would be compared to the Raman spectra, and a multivariate data analysis (MVDA) model would be developed. You may need to download version 2.0 now from the Chrome Web Store. The obtained spectra were preprocessed, and differential spectra were calculated by subtraction of the core spectrum from each inline spectrum. In biopharmaceutical industry, inline process control, particularly using Raman spectroscopy, provides substantial benefits to ensure maximum product yield and to increase the level of process automation. The equipment used was a fiber-optic immersion probe with a 785-nm laser operating at the 300 mW power setting. This list continues to grow as more industries learn about the value of process Raman spectroscopy. Pinkard, D.J. #raman #manufacturingtechnology #inlineanalysis, By Brian Marquardt, Ph.D, MarqMetrix Founder & CEO,  and Bharat Mankani, Ph.D, MarqMetrix Application Specialist. Another way to prevent getting this page in the future is to use Privacy Pass. Reducing the cost of ownership is imperative when using Raman spectroscopy for continuous process analysis and control. Published in the November 2020 issue of, Raman spectroscopy is no longer reserved for just laboratory use. Completing the CAPTCHA proves you are a human and gives you temporary access to the web property. Trace gas and dynamic process monitoring by Raman spectroscopy in metal-coated hollow glass fibres. Raman can also be used as an online–inline optical sensor, bringing analysis right to the production line (for example, a conveyor belt, tablet coater, or wet milling operation). 2.2. In this example, process Raman spectroscopy was used in the analysis of the kinetics of decomposition of formic acid in sub- and supercritical water. (4) “What is Multivariate Analysis?” Camo Analytics (Oslo, Norway, 2019). Sci. MarqMetrix Process Raman systems make this easier, faster and repeatable: http://ow.ly/hDUo50CWoNs Since its discovery in the 1920s, Raman spectroscopy has revolutionized process analysis with its nondestructive nature and ability to measure sample composition. The difficulty lies in measuring this property directly in the process via on or inline technologies. (5) “Process analytical technologies: a unique data management challenge,” Optimal Industrial Automation Ltd. (Bristol, United Kingdom, 2012). For instance, using an immersion Raman probe eliminates the need for toxic samples to be extracted from the process and analyzed in the laboratory, significantly reducing exposure. Installation of a solid-state process Raman spectroscopy system is straightforward, and it does not require a sophisticated sampling system. What is the optimal way to manage process variability? Our company was founded in 2009 and is now co-funded by the Horizon 2020 program of … A cross-validation technique was used to ensure realistic error statistics were calculated. Bioanal. Furthermore, selecting the appropriate equipment is of the utmost importance when taking Raman spectroscopy out of a laboratory setting. A Raman signal is straightforward to interpret: The distribution of the spectral peaks describes the molecule’s composition while the signal intensity correlates linearly with concentration. Water becomes a unique fluid above its thermodynamic critical point (374 °C, 22.1 MPa), but there are very few tools that can study the decomposition of organic waste at these temperatures and pressures. The technique is nondestructive and can measure samples in real-time, making it an effective process analysis tool. Businesses desire production insights to facilitate real-time decisions that may ultimately give them an advantage over their competitors. Once installed, process Raman spectroscopy has considerable operator health and safety benefits because samples are no longer required to be extracted from the manufacturing process and transported to the laboratory for analysis. An example is trimethyl aluminum (TMA), a highly sensitive and reactive chemical compound. Quantitative analyses utilizing Raman spectroscopy are of increasing importance in the manufacturing industry for online or inline process control. At temperatures above 430 °C, formic acid decomposed too rapidly to quantify kinetic rates accurately. The second is the transformation of organic waste into alternate fuels. Science Translational Medicine has called TMA reactions “the most notorious fireball in all of chemistry” (7). Raman spectroscopy is commonly used in chemistry to provide a structural fingerprint by which molecules can be identified. (8) B. Zachhuber, G. Ramer, A. Hobro, E. T.H. The Analyst 2015, 140 (3) , 854-859. To control product quality during HME without process interruption, integration of inline analytical technology is critical. The experimental conditions of the supercritical water reactor and the gasified formic acid were as follows: Continuous temperatures hovered between 300 and 430 °C; a pressure of 25 MPa was maintained; residence times stayed between 4 and 65 s; and a feedstock concentration was set at 3.6 wt%. 10/23/2020 10:00 AM EST. Such analysis is possible using a Raman microscope. A semi-manual fluorescent background subtraction method was used for consistent spectral processing. J. Hydrog. Technical requirements to look for are a 785-nm laser wavelength for the right balance between signal strength and reduced fluorescence, open platform communications (OPC)–compatibility, and the ability to interface with chemometrics process software, all of which are important considerations for process analysis. Process operators then extract samples at set times and send them to the laboratory for analysis. When applied in manufacturing processes, Raman spectroscopy provides results in seconds, transmitted to an advanced process control (APC) system to increase process understanding and efficiency, ensuring product quality and consistency. The specificity of spectra, in conjunction with standard chemometric methods, enables selected monitoring, control, and … Esmonde-White K, Cuellar M, Uerpmann C, Lenain B, Lewis I. Raman spectroscopy as a … Manufacturers handling hazardous materials in their production facilities face unique analytical challenges when it comes to the collection of samples for laboratory analysis. The immersion probe’s sapphire ball lens focuses the laser into the sample flow. Results are updated once per minute, and process operators make key decisions that ensure product quality and consistency while reducing cycle times (Figure 3). Different exciting lasers may be used to excite a microscopic sample at different wavelengths so that the Raman microspectrometer can collect and analyze the vibrational spectra. These substances do not travel well and could potentially be dangerous to the person sampling or handling them (6). The DoE sample set used for calibration was stored in glass vials under nitrogen, and Raman spectra were collected using a contact-free probe measuring through the vial. There is little to no sample preparation required when using Raman spectroscopy. Process operators set control limits, alarms, and warnings on the process control system. It can transform organic waste, such as biomass, into hydrogen-rich syngas. Advancements in the stability and portability of certain Raman systems demonstrate that this technology can be moved out of the traditional laboratory and installe. More recently, Raman spectroscopy has been successfully applied to inline monitoring of animal cell bioreactor cultures [63, 65, 67]. Organometallics are manufactured in large volumes with very high purity, which presents major analytical obstacles, because sample handling is hazardous (8). Still today the usage is limited to a small application area. Decisions to adjust operational parameters are made based using these well-timed insights. Organometallic compounds like TMA constitute an extensive group of substances that have played a significant role in chemistry development. The results show that in-line Raman spectroscopy is a viable technique for monitoring the progress of particle size reduction during wet media milling. Vibrational spectroscopy (Raman, NIR and FT-IR) is often employed and used for real-time measurements because of the non-destructive and rapid nature of these analytical techniques. Figure 2 shows the statistical results for the cross-validation with both a regression line (Figure 2a) and target line alongside the plot of error versus the number of PLS factors (Figure 2b). The simplicity of this data-rich qualitative and quantitative information makes Raman spectroscopy an attractive process analytical technology (PAT) tool. Cloudflare Ray ID: 60afaa12af130820 Performance & security by Cloudflare, Please complete the security check to access. Chem. 5(47), 1–14 (2018). ProCellics™ – First In-Line and Real-Time Bioprocess Raman Analyzer Grenoble, FRANCE, November 14, 2017 – ProCellics™, first In-Line and Real-Time Bioprocess Raman Analyzer, is a RESOLUTION Spectra Systems product. ProCellics™ is the first GMP Bioprocess Raman Analyzer for the biopharmaceutical industry fully dedicated to in-line monitoring of the critical parameters and quality attributes in real-time, in order to maintain an optimal development of cultures in bioreactors and to better control downstream processes. DOI: 10.1039/C4AN00913D. In the case of supercritical water gasification (SCWG) of organics, Raman spectroscopy is the only technology that can effectively measure the required CPPs (4). Raman spectroscopy was fully understood and accepted for process industrial applications . Continuous measurements from infrared spectrometers are used for obtaining reaction profiles to calculate reaction rates. Baeyens, J. Pharm. In this work 26 proteins of different structure, function and properties are investigated by Raman spectroscopy with 488, 532 and 1064 nm laser lines. Med, June 2, 2020. It produces a unique spectral fingerprint that identifies the chemical composition and molecular structure of materials. There were minor changes to integration time because longer lengths of optical fiber were used, and the analysis was subsequently performed using third-party process control software (Camo Analytics Process Pulse II) for model execution, visualization, and transmission of results via fieldbus to the plant process control system. Raman spectroscopy was used to predict the application of coatings on three different tablet cores using PLS regression. As a result, Raman is now a powerful measurement solution for research and development, manufacturing, and quality control. In addition, the evolution of Raman spectrometers and probes reveals that Raman equipment has long had a reputation for being expensive, cumbersome, and difficult to use. For the spectra collected in this study, indirect hard modeling (IHM) was used to extract species concentration data. A Raman measurement can be collected in seconds for most organic substances. This action allows calibration without an extensive set of known mixture spectra that are challenging to produce for a two-phase effluent stream of a reacting system. A total of 10 experimental conditions were tested in a day, demonstrating the potential for expediting future gasification studies with more complex model compounds. This was a key theme at the 8th Symposium on Continuous Flow Reactor Technology for Industrial Applications held in Delft in 2016, and many of our customers have asked what techniques New Raman spectroscopy applications are emerging in non-traditional fields because of advancements in easy-to-use commercial Raman spectroscopy instrumentation (1). The second is the transformation of organic waste into alternative fuels, where monitoring the reaction offline at extreme temperatures and pressures is dangerous, time-consuming, and expensive. such as a smartphone. One strategy is to use a sensitive, spectroscopic inline measurement for process monitoring and control during the manufacturing process. Kaiser has been a leader of in situ monitoring and control in bioprocessing since 2007 because of our robust in situ Raman solutions and expertise in life sciences from R&D to GMP.. References. Inline liquid measurements are critical to chemical, petrochemical, and small molecule pharmaceutical processes. Gorman, E.G. Acquisition parameters set the integration time to 230 mS and the laser power to 450 mW. For in-line measurements, a fibre-optic Raman Dynisco probe was connected into the die head (Fig. This combination ensures maximum sample variance with a minimum number of samples, and a purity range of 90 to 99.9%. Is multivariate analysis? ” Camo Analytics ( Oslo, Norway, 2019 ) a... Identical probe with a laser onto a sample measured in-situ and processes can be optimized.. Detailed band assignment an attractive process analytical technology ( 9,10 ) monitor two lines. Inline ’ manufacturing for sensor solutions application note demonstrates the use of the importance. Well-Timed insights handling challenges, sample extraction and handling, especially when hazardous materials in their production facilities unique... 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